Direct Profiling of Environmental Microbial Populations by Thermal Dissociation Analysis of Native rRNAs Hybridized to Oligonucleotide Microarrays

Authors

Said El Fantroussi
Hidetoshi Urakawa
Anne E. Bernhard
John J. Kelly, Loyola University ChicagoFollow
Peter A. Noble
H Smidt
G M. Yershov
David A. Stahl

Document Type

Article

Publication Date

4-2003

Publication Title

Applied and Environmental Microbiology

Volume

69

Issue

4

Abstract

Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.

Comments

Author Posting. © American Society for Microbiology, 2003. This article is posted here by permission of the American Society for Microbiology for personal use, not for redistribution. The article was published in Applied and Environmental Microbiology, Volume 69, Issue 4, April 2003. http://dx.doi.org/ 10.1128/AEM.69.4.2377–2382.2003

Recommended Citation

Fantroussi, SE, H Urakawa, AE Bernhard, JJ Kelly, PA Noble, H Smidt, GM Yershov, and DA Stahl. "Direct Profiling of Environmental Microbial Populations by Thermal Dissociation Analysis of Native rRNAs Hybridized to Oligonucleotide Microarrays." Applied and Environmental Microbiology 69(4), 2003.

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Copyright Statement

© American Society for Microbiology, 2003.