Date of Award

2012

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

Abstract

The purpose of this project was to develop a better map of the alpha 21-II region of human chromosome 21 in regards to size and primary sequence of the five alphoid clusters that comprise the region and to explore the evolutionary relationships that exist within and between these clusters. Several HC21 BACs were identified as containing strong sequence identities to alpha 21-II clones and were mapped to various alpha 21-II clusters. It was revealed that both monomeric and HOR alphoid clusters can be found in the alpha 21-II region. Phylogenetic analysis revealed separate evolutionary histories for the monomeric and HOR sequences. The size of each of the alpha 21-II clusters was estimated. This study finds that HC21 has a different organization of alphoid clusters compared to other characterized chromosomes. These differences are likely the result of interchromosomal exchanges between the acrocentric chromosomes.

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Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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