Improved Detection of Bartonella DNA in Mammalian Hosts and Arthropod Vectors by Real-Time PCR Using the NADH Dehydrogenase Gamma Subunit (nuoG)

Authors

James M. Colborn
Michael Y. Kosoy
Vladimir L. Motin
Maxim V. Telepnev
Gustavo Valbuena
Khin S. Myint
Yuri Fofanov
Catherine Putonti, Loyola University ChicagoFollow
Chen Feng
Leonard Peruski

Document Type

Article

Publication Date

2010

Publication Title

Journal of Clinical Microbiology

Volume

48

Issue

12

Publisher Name

American Society for Microbiology

Abstract

We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit (nuoG) primer set that is sensitive and specific enough to detect a diverse number of Bartonella species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms.

Comments

Author Posting. © American Society for Microbiology, 2010. This article is posted here by permission of the American Society for Microbiology for personal use, not for redistribution. The article was published in the Journal of Clinical Microbiology, Volume 48, Issue 12, March 2010, http://dx.doi.org/10.1128/JCM.00470-10

Recommended Citation

Colborn, JM, MY Kosoy, VL Motin, MV Telepnev, G Valbuena, KS Myint, Y Fofanov, C Putonti, C Feng, and L Peruski. "Improved Detection of Bartonella DNA in Mammalian Hosts and Arthropod Vectors by Real-Time PCR Using the NADH Dehydrogenase Gamma Subunit (nuoG)." Journal of Clinical Microbiology 48(12), 2010.

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Copyright Statement

© American Society for Microbiology, 2010.