Major
Biology
Anticipated Graduation Year
2026
Access Type
Open Access
Abstract
This study investigates the role of exon 2 in the zebrafish col2a1a gene, a paralog of the human COL2A1 gene essential for connective tissue and skeletal development. Exon 2 encodes the von Willebrand Factor Type C (VWF-C) domain, which binds TGF-β ligands involved in embryonic dorsoventral patterning. Using CRISPR-Cas9, we aim to delete exon 2 and prevent expression of the embryonic splice variant (v1), forcing premature use of the post-embryonic variant (v2). We hypothesize that loss of the VWF-C domain will disrupt TGF-β signaling, leading to defects in embryonic development and vertebral formation.
Faculty Mentors & Instructors
Rodney Dale, PhD, Biology; Megan Stropus, Graduate Student, Biology; Xela Bui; Mia Cortizo, Luke Hosek, Fiona Joslin
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Elucidating the Function of an Evolutionarily Conserved Embryonic Splice Variant of Type II Collagen During Vertebrate Development
This study investigates the role of exon 2 in the zebrafish col2a1a gene, a paralog of the human COL2A1 gene essential for connective tissue and skeletal development. Exon 2 encodes the von Willebrand Factor Type C (VWF-C) domain, which binds TGF-β ligands involved in embryonic dorsoventral patterning. Using CRISPR-Cas9, we aim to delete exon 2 and prevent expression of the embryonic splice variant (v1), forcing premature use of the post-embryonic variant (v2). We hypothesize that loss of the VWF-C domain will disrupt TGF-β signaling, leading to defects in embryonic development and vertebral formation.