Document Type

Article

Publication Date

6-11-2015

Publication Title

PLOS One

Volume

10

Issue

6

Pages

1-21

Publisher Name

Public Library of Science

Abstract

Control of stem cell migration and differentiation is vital for efficient stem cell therapy. Literature reporting electric field–guided migration and differentiation is emerging. However, it is unknown if a field that causes cell migration is also capable of guiding cell differentiation— and the mechanisms for these processes remain unclear. Here, we report that a 115 V/m direct current (DC) electric field can induce directional migration of neural precursor cells (NPCs). Whole cell patching revealed that the cell membrane depolarized in the electric field, and buffering of extracellular calcium via EGTA prevented cell migration under these conditions. Immunocytochemical staining indicated that the same electric intensity could also be used to enhance differentiation and increase the percentage of cell differentiation into neurons, but not astrocytes and oligodendrocytes. The results indicate that DC electric field of this specific intensity is capable of promoting cell directional migration and orchestrating functional differentiation, suggestively mediated by calcium influx during DC field exposure.

Comments

Author Posting. © Zhao et al., 2015. This article is posted here by permission of the authors for personal use, not for redistribution. The article was published in PLOS One, Volume 10, Issue 6, 2015, http://dx.doi.org/10.1371/journal.pone.0129625

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Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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