Document Type
Article
Publication Date
3-2-2016
Publication Title
PLoS ONE
Volume
11
Issue
3
Pages
1-23
Abstract
Oxygen-glucose deprivation (OGD) leads to depression of evoked synaptic transmission, for which the mechanisms remain unclear. We hypothesized that increased presynaptic [Ca2+]i during transient OGD contributes to the depression of evoked field excitatory postsynaptic potentials (fEPSPs). Additionally, we hypothesized that increased buffering of intracellular calcium would shorten electrophysiological recovery after transient ischemia. Mouse hippocampal slices were exposed to 2 to 8 min of OGD. fEPSPs evoked by Schaffer collateral stimulation were recorded in the stratum radiatum, and whole cell current or voltage clamp recordings were performed in CA1 neurons. Transient ischemia led to increased presynaptic [Ca2+]i, (shown by calcium imaging), increased spontaneous miniature EPSP/Cs, and depressed evoked fEPSPs, partially mediated by adenosine. Buffering of intracellular Ca2+ during OGD by membrane-permeant chelators (BAPTA-AM or EGTA-AM) partially prevented fEPSP depression and promoted faster electrophysiological recovery when the OGD challenge was stopped. The blocker of BK channels, charybdotoxin (ChTX), also prevented fEPSP depression, but did not accelerate post-ischemic recovery. These results suggest that OGD leads to elevated presynaptic [Ca2+]i, which reduces evoked transmitter release; this effect can be reversed by increased intracellular Ca2+ buffering which also speeds recovery.
Recommended Citation
Jalini, Shirin; Ye, Hui; Tonkikh, Alexander A.; Charlton, Milton P.; and Carlen, Peter L.. Raised Intracellular Calcium Contributes to Ischemia-Induced Depression of Evoked Synaptic Transmission. PLoS ONE, 11, 3: 1-23, 2016. Retrieved from Loyola eCommons, Biology: Faculty Publications and Other Works, http://dx.doi.org/10.1371/journal.pone.0148110
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Statement
© Jalini S, Ye H, Tonkikh AA, Charlton MP, Carlen PL, 2016.
Comments
Author Posting © Jalini S, Ye H, Tonkikh AA, Charlton MP, Carlen PL, 2016. This article is posted here by permission of PLOS for personal use, not for redistribution. The article was published in PLoS ONE, Vol. 11, Iss. 3, March 2006, http://dx.doi.org/10.1371/journal.pone.0148110