Document Type

Article

Publication Date

2-6-2018

Publication Title

eLife

Volume

7

Pages

1-26

Abstract

Regenerative therapy for degenerative spine disorders requires the identification of cells that can slow down and possibly reverse degenerative processes. Here, we identify an unanticipated wound-specific notochord sheath cell subpopulation that expresses Wilms Tumor (WT) 1b following injury in zebrafish. We show that localized damage leads to Wt1b expression in sheath cells, and that wt1b+cells migrate into the wound to form a stopper-like structure, likely to maintain structural integrity. Wt1b+sheath cells are distinct in expressing cartilage and vacuolar genes, and in repressing a Wt1b-p53 transcriptional programme. At the wound, wt1b+and entpd5+ cells constitute separate, tightly-associated subpopulations. Surprisingly, wt1b expression at the site of injury is maintained even into adult stages in developing vertebrae, which form in an untypical manner via a cartilage intermediate. Given that notochord cells are retained in adult intervertebral discs, the identification of novel subpopulations may have important implications for regenerative spine disorder treatments.

Comments

Author Posting. © The Authors 2018. This article is posted here by permission of Rodney M. Dale for personal use, not for redistribution. The article was published in eLife, vol. 7, 2018, https://doi.org/10.7554/eLife.30657.001

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

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