Document Type

Article

Publication Date

6-11-2018

Publication Title

Journal of General Virology

Volume

99

Issue

8

Pages

1141-1146

Publisher Name

Microbiology Society

Abstract

Viruses are the most abundant component of the human microbiota. Recent evidence has uncovered a rich diversity of viruses within the female bladder, including both bacteriophages and eukaryotic viruses. We conducted whole-genome sequencing of the bladder microbiome of 30 women: 10 asymptomatic ‘healthy’ women and 20 women with an overactive bladder. These metagenomes include sequences representative of human, bacterial and viral DNA. This analysis, however, focused specifically on viral sequences. Using the bioinformatic tool virMine, we discovered sequence fragments, as well as complete genomes, of bacteriophages and the eukaryotic virus JC polyomavirus. The method employed here is a critical proof of concept: the genomes of viral populations within the low-biomass bladder microbiota can be reconstructed through whole-genome sequencing of the entire microbial community.

Comments

Author Posting. © The Authors 2018. This article is posted here by permission of the Microbiology Society for personal use, not for redistribution. The article was published in the Journal of General Virology, 2018, https://dx.doi.org/10.1099/jgv.0.001097

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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