Document Type
Article
Publication Date
4-10-2024
Publication Title
PLOS One
Volume
19
Issue
4
Pages
1-15
Publisher Name
PLOS
Abstract
RiSE study aims to evaluate a race-based stress-reduction intervention as an effective strategy to improve coping and decrease stress-related symptoms, inflammatory burden, and modify DNA methylation of stress response-related genes in older AA women. This article will describe genomic analytic methods to be utilized in this longitudinal, randomized clinical trial of older adult AA women in Chicago and NYC that examines the effect of the RiSE intervention on DNAm pre- and post-intervention, and its overall influence on inflammatory burden. Salivary DNAm will be measured at baseline and 6 months following the intervention, using the Oragene-DNA kit. Measures of perceived stress, depressive symptoms, fatigue, sleep, inflammatory burden, and coping strategies will be assessed at 4 time points including at baseline, 4 weeks, 8 weeks, and 6 months. Genomic data analysis will include the use of pre-processed and quality-controlled methylation data expressed as beta (β) values. Association analyses will be performed to detect differentially methylated sites on the targeted candidate genes between the intervention and non-intervention groups using the Δβ (changes in methylation) with adjustment for age, health behaviors, early life adversity, hybridization batch, and top principal components of the probes as covariates. To account for multiple testing, we will use FDR adjustment with a corrected p-value of < 0.05 regarded as statistically significant. To assess the relationship between inflammatory burden and Δβ among the study samples, we will repeat association analyses with the inclusion of individual inflammation protein measures. ANCOVA will be used because it is more statistically powerful to detect differences.
Recommended Citation
Taylor, Jacquelyn Y.; Jones-Patten, Alexandria; Prescott, Laura; Potts-Thompson, Stephanie; Joyce, Cara; Tayo, Bamidele; and Saban, Karen, "The race-based stress reduction intervention (RiSE) study on African American women in NYC and Chicago: Design and methods for complex genomic analysis" (2024). Biostatistics Collaborative Core: Faculty Publications and Other Works. 4.
https://ecommons.luc.edu/biostatistics_facpubs/4
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Copyright Statement
© The Author(s), 2024.
Comments
Author Posting © The Author(s), 2024. This article is posted here by permission of PLOS for personal use and redistribution. This article was published open access in PLOS One, Vol. 19, Iss. 4, (April 10, 2024), https://doi.org/10.1371/journal.pone.0295293.