Parkinson Disease-Associated Mutation R1441H in LRRK2 Prolongs the “Active State” of its GTPase Domain

Authors

Jingling Liao, Stark Neurosciences Institute, Indiana University
Chun-Xiang Wu, Stark Neurosciences Institue, Indiana University
Christopher Burlak, Indiana University
Sheng Zhang, Indiana University
Dali Liu, Loyola University ChicagoFollow

Document Type

Article

Publication Date

3-3-2014

Publication Title

Proceedings of the National Academy of Sciences of the United States of America

Volume

111

Issue

11

Pages

4055-4060

Publisher Name

National Academy of Sciences

Abstract

Mutation in leucine-rich-repeat kinase 2 (LRRK2) is a common cause of Parkinson disease (PD). A disease-causing point mutation R1441H/G/C in the GTPase domain of LRRK2 leads to overactivation of its kinase domain. However, the mechanism by which this mutation alters the normal function of its GTPase domain [Ras of complex proteins (Roc)] remains unclear. Here, we report the effects of R1441H mutation (Roc_R1441H) on the structure and activity of Roc. We show that Roc forms a stable monomeric conformation in solution that is catalytically active, thus demonstrating that LRRK2 is a bona fide self-contained GTPase. We further show that the R1441H mutation causes a twofold reduction in GTPase activity without affecting the structure, thermal stability, and GDP-binding affinity of Roc. However, the mutation causes a twofold increase in GTP-binding affinity of Roc, thus suggesting that the PD-causing mutation R1441H traps Roc in a more persistently activated state by increasing its affinity for GTP and, at the same time, compromising its GTP hydrolysis.

Identifier

24591621

Comments

Author Posting. © National Academy of Sciences, 2014. This article is posted here by permission of National Academy of Sciences for personal use, not for redistribution. It was published in Proceedings of the National Academy of Sciences of the United States of America, 111 (11), (2014). http://dx.doi.org/10.1073/pnas.1323285111

Recommended Citation

Jingling Liao, Chun-Xiang Wu, Christopher Burlak, Sheng Zhang, Heather Sahm, Mu Wang, Zhong-Yin Zhang, Kurt W. Vogel, Mark Federici, Steve M. Riddle, R. Jeremy Nichols, Dali Liu, Mark R. Cookson, Todd A. Stone, and Quyen Q. Hoang. Parkinson disease-associated mutation R1441H in LRRK2 prolongs the “active state” of its GTPase domain. Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 11 (2014).

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© National Academy of Sciences 2014.