Date of Award

Fall 9-5-2025

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Catherine Putonti

Abstract

This thesis investigates the microbial diversity of Staphylococcus epidermidis (S. epidermidis) across various anatomical sites in the human body. While traditionally viewed as a benign skin microbe, S. epidermidis also inhabits the urinary, gastrointestinal, and respiratory tracts, raising questions about its genetic variation and adaptability in different environments. The research aims to compare strains of S. epidermidis isolated from the oral cavity, nostrils, urinary tract, and skin of healthy individuals to determine if they share a common ancestor or if they evolved independently in response to specific environmental factors. The study comprises three main aims. Aim 1 focuses on assessing the prevalence of S. epidermidis across the selected sites by collecting and purifying samples from 50 healthy female participants, using selective media for isolation and PCR for identification. Aim 2 involves comparative genetic analysis of the isolated strains to identify genotypic differences, pan-genome composition, and genes associated with niche adaptation. Aim 3 aims to analyze the microbial composition of the samples through metagenomic sequencing, particularly examining co-occurring species with S. epidermidis. The proposal highlights the intellectual merit of addressing gaps in current microbiome research by examining S. epidermidis beyond the skin. It emphasizes the significance of host-microbe interactions and the potential for S. epidermidis to act as both a benign and pathogenic organism. The broader impacts include engaging undergraduates in research and enhancing educational experiences through hands-on learning in bioinformatics and metagenomics, contributing to a deeper understanding of the role of S. epidermidis in human health.

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