Date of Award
2021
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Molecular Biology
Abstract
Variable sensitivity to ultraviolet B radiation (UVB) in humans is apparent from individual responses to UVB-induced sunburn, tanning and skin cancer. This variation is largely credited to variability in skin pigmentation; however we have observed heterogeneity in the UV sensitivity in cultured normal human epidermal keratinocytes (NHEK). Our goal is to elucidate gene expression profiles that either exist intrinsically or are present as a result of adaptation that can help to explain variability in sensitivity to UV in the normal human population.In our study, we observed that HaCaT cells are able to transiently adapt to have increased UV resistance in response to chronic low dose UV (CLUV) treatment, however, this increased resistance was not sustained. Additionally, we have shown that NHEKs exhibit cell-intrinsic variation in UV sensitivity and that there are district gene expression patterns that are associated with variable sensitivity to UV radiation.
Recommended Citation
Richenberger, Casey, "Adaptive and Intrinsic Responses of Human Keratinocytes to Ultraviolet Radiation" (2021). Master's Theses. 4397.
https://ecommons.luc.edu/luc_theses/4397
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Copyright Statement
Copyright © 2021 Casey Richenberger
