Ribosomal/Nucleolar Stress Induction Regulates Tert-Butyl Hydroperoxide (tBHP) Mediated Oxidative Stress in Anopheles Gambiae Midguts

Authors

Brian B. Tarimo, Nelson Mandela-African Institution of Science and Technology
Bernadette A. Hritzo, Johns Hopkins Bloomberg School of Public Health
Henry Chun Hin Law, University of Florida
Dingyin Tao, Johns Hopkins Bloomberg School of Public Health
Rebecca Pastrana-Mena, Johns Hopkins Bloomberg School of Public Health
Stefan M. Kanzok, Loyola University ChicagoFollow
Joram J. Buza, Nelson Mandela-African Institution of Science and Technology
Rhoel R. Dinglasan, Nelson Mandela-African Institution of Science and Technology

Document Type

Article

Publication Date

3-29-2019

Publication Title

BMC Research Notes

Volume

12

Publisher Name

Springer Nature

Abstract

Objective

A fundamental understanding of redox homeostasis in Anopheles gambiae midgut cells under different oxidative conditions is missing. Such knowledge can aid in the development of new malaria transmission-blocking strategies aimed at disrupting natural homeostatic processes in the mosquito during Plasmodium parasite uptake (i.e. blood feeding). The aim of this study was to understand how the An. gambiae midgut regulates oxidative stress to reactive oxygen species (ROS), especially to a potent ROS-inducer such as tert-Butyl hydroperoxide (tBHP).

Results

Initial studies using quantitative immunoblot indicated that the expression of the classical antioxidant protein An. gambiae thioredoxin-1 (AgTrx-1) remained unchanged across challenges with different concentrations of tBHP suggesting that additional mechanisms to regulate ROS may be involved. We therefore conducted a global proteomic survey, which revealed that An. gambiae midguts under low (50 μM) and high (200 μM) tBHP concentrations were enriched in proteins indicative of ribosomal/nucleolar stress. Ribosomal stress is an inherent cellular response to an imbalance in ribosomal proteins (RPs) due to cellular stress such as oxidative stress. Our data suggest that ribosomal/nucleolar stress is the primary cellular response in An. gambiae midguts under tBHP challenge. Considering these results, we discuss harnessing the ribosomal stress response as a potential malaria transmission-blocking strategy.

Comments

Author Posting © The Authors, 2019. This article is posted here by permission of the Authors for personal use, not for redistribution. The article was published in BMC Research Notes, Volume 12, Article 182, March 2019, https://doi.org/10.1186/s13104-019-4196-1

Recommended Citation

Tarimo, Brian B.; Hritzo, Bernadette A.; Chun Hin Law, Henry; Tao, Dingyin; Pastrana-Mena, Rebecca; Kanzok, Stefan M.; Buza, Joram J.; and Dinglasan, Rhoel R.. Ribosomal/Nucleolar Stress Induction Regulates Tert-Butyl Hydroperoxide (tBHP) Mediated Oxidative Stress in Anopheles Gambiae Midguts. BMC Research Notes, 12, : , 2019. Retrieved from Loyola eCommons, Biology: Faculty Publications and Other Works, http://dx.doi.org/10.1186/s13104-019-4196-1

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright Statement

© The Authors, 2019.