Major

Forensic Science

Anticipated Graduation Year

2021

Access Type

Restricted Access

Abstract

Quantitation of human DNA is a critical but expensive step in forensic DNA analysis. Quantifiler® Duo and Quantifiler® Trio are two commercial kits commonly used. Reduction in reaction volume holds a large potential for cost savings for laboratories. This work evaluated reducing reaction volume to ~25% of manufacturer's protocol. Both kits returned reproducible quantitation results in reduced volumes and provided comparable results between the two reaction volumes. Elevated or IPC undetected IPC CT in reduced Quantifiler® Duo reaction volume does not predict inhibited STR amplification. Degraded samples returned comparable results in reduced and full volume reactions in Quantifiler® Trio.

Faculty Mentors & Instructors

Wendy Gruhl, Lecturer, Forensic Science Program & Department of Biology

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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Evaluation of Reduced Reaction Volumes for Quantifiler® Duo and Quantifiler® Trio

Quantitation of human DNA is a critical but expensive step in forensic DNA analysis. Quantifiler® Duo and Quantifiler® Trio are two commercial kits commonly used. Reduction in reaction volume holds a large potential for cost savings for laboratories. This work evaluated reducing reaction volume to ~25% of manufacturer's protocol. Both kits returned reproducible quantitation results in reduced volumes and provided comparable results between the two reaction volumes. Elevated or IPC undetected IPC CT in reduced Quantifiler® Duo reaction volume does not predict inhibited STR amplification. Degraded samples returned comparable results in reduced and full volume reactions in Quantifiler® Trio.