Major
Physics
Anticipated Graduation Year
2022
Access Type
Open Access
Abstract
This study developed a single-molecule-based assay to track the looping of dsDNA molecules. DNA encodes our genetic information through a combination of four nucleotides; base pairing forms dsDNA molecules in a double-helical form. The genome achieves a three-dimensional architecture; the mechanical properties of dsDNA are sensitive to sequence, resulting in genomic misfolding linked to many disorders. Modified dsDNA molecules were constructed that permit observation of dsDNA looping by fluorescence microscopy. The assay shows that DNA looping is a highly dynamic process and is sensitive to ionic conditions and molecular crowding. Future work will probe how defects alter the looping behavior.
Faculty Mentors & Instructors
Dr. Brian Cannon, Associate Professor, Physics
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Effects of DNA looping behavior using smFRET
This study developed a single-molecule-based assay to track the looping of dsDNA molecules. DNA encodes our genetic information through a combination of four nucleotides; base pairing forms dsDNA molecules in a double-helical form. The genome achieves a three-dimensional architecture; the mechanical properties of dsDNA are sensitive to sequence, resulting in genomic misfolding linked to many disorders. Modified dsDNA molecules were constructed that permit observation of dsDNA looping by fluorescence microscopy. The assay shows that DNA looping is a highly dynamic process and is sensitive to ionic conditions and molecular crowding. Future work will probe how defects alter the looping behavior.
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