Major
Forensic Science
Access Type
Open Access
Abstract
Quantifiler Trio is commonly used in forensic DNA analysis to evaluate samples for the presence of male-female mixtures, degradation, and inhibition. This work explores a modified protocol with reduced reaction volumes as it relates to inhibition. Samples with constant DNA amounts and varying levels of humic acid were created and evaluated. Selected samples were amplified with Globalfiler to assess if inhibition in the modified Quantifiler Trio protocol correlates to inhibition during STR amplification.
Faculty Mentors & Instructors
Wendy Gruhl, MS, Forensic Science
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Quantifiler Trio Reduced Reaction Volume and Inhibited Samples
Quantifiler Trio is commonly used in forensic DNA analysis to evaluate samples for the presence of male-female mixtures, degradation, and inhibition. This work explores a modified protocol with reduced reaction volumes as it relates to inhibition. Samples with constant DNA amounts and varying levels of humic acid were created and evaluated. Selected samples were amplified with Globalfiler to assess if inhibition in the modified Quantifiler Trio protocol correlates to inhibition during STR amplification.