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Major
Biology
Anticipated Graduation Year
2022
Access Type
Open Access
Abstract
Numerous genetic disorders such as fragile X syndrome, spinocerebellar ataxia, ALS, and Huntington’s disease arise from repetitive DNA. Repeats in a DNA sequence can cause altered base pairing in single-stranded domains, which can lead to the formation of larger structures, such as hairpins. These structures can disrupt genome integrity and cell functioning. Detection of hairpin formation can be useful in understanding the structural dynamics behind them and possibly the development of treatments with preventative or reductive effects. This project sought to develop a triplex-forming strategy to identify the presence and number of repeat hairpins in double-stranded DNA.
Faculty Mentors & Instructors
Dr. Brian Cannon, Department of Physics; Gilberto Garcia, Research Assistant, Department of Physics
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Detection of Multiple Hairpins in a DNA Construct
Numerous genetic disorders such as fragile X syndrome, spinocerebellar ataxia, ALS, and Huntington’s disease arise from repetitive DNA. Repeats in a DNA sequence can cause altered base pairing in single-stranded domains, which can lead to the formation of larger structures, such as hairpins. These structures can disrupt genome integrity and cell functioning. Detection of hairpin formation can be useful in understanding the structural dynamics behind them and possibly the development of treatments with preventative or reductive effects. This project sought to develop a triplex-forming strategy to identify the presence and number of repeat hairpins in double-stranded DNA.