Major
Biology
Anticipated Graduation Year
2023
Access Type
Open Access
Abstract
This project’s intention is to understand the role of Hedgehog signaling in craniofacial development in the lizard Anolis sagrei. There’s extensive research regarding craniofacial development of avian and mammalian species but there’s a gap in knowledge regarding lizards. We hypothesized that the knockdown of Hedgehog signaling in A. sagrei will result in disrupted formation to the midline facial structures, specifically the premaxilla. Stage matched embryos were collected and administered different concentrations of the chemical cyclopamine to knock down signaling in the developing embryo at different stages. To assess the morphology, we acquired frontal and lateral microscopic and micro-CT scanned images.
Faculty Mentors & Instructors
Dr. Thomas Sanger, Professor, Department of Biology
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Knockdown of Hedgehog signaling pathway contributes to midline craniofacial development and facial elongation in the lizard Anolis sagrei
This project’s intention is to understand the role of Hedgehog signaling in craniofacial development in the lizard Anolis sagrei. There’s extensive research regarding craniofacial development of avian and mammalian species but there’s a gap in knowledge regarding lizards. We hypothesized that the knockdown of Hedgehog signaling in A. sagrei will result in disrupted formation to the midline facial structures, specifically the premaxilla. Stage matched embryos were collected and administered different concentrations of the chemical cyclopamine to knock down signaling in the developing embryo at different stages. To assess the morphology, we acquired frontal and lateral microscopic and micro-CT scanned images.
Comments
Naaz Khan is presenting her work on the basis of being a member of the Mulcahy Scholars Program. Greta Keller is presenting her work on the basis of the Biology Research Fellowship.