Major
Biology
Anticipated Graduation Year
2022
Access Type
Open Access
Abstract
Our lab has demonstrated that PhLP-3 is redox active. We already confirmed that PhLP-3 homologues are present in evolutionary model organisms and possess enzymatic activity. I have been working on expressing and purifying the PhLP-3 protein in Drosophila. I hypothesize that DmPhLP-3 (also referred to as CG4511) plays a significant role in the development of germ line cells. I performed a spin purification of DmPhLP-3 to ensure that the protein was being expressed. I performed a large-scale stepwise purification using a gravity column. I successfully cloned, expressed, and purified truncated version of Drosophila PhLP-3 (DmPhLP-3 ΔH) in the stepwise purification.
Faculty Mentors & Instructors
Stefan Kanzok, PhD, Department of Biology
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Expression and Purification of Drosophila Phosducin-like Protein 3
Our lab has demonstrated that PhLP-3 is redox active. We already confirmed that PhLP-3 homologues are present in evolutionary model organisms and possess enzymatic activity. I have been working on expressing and purifying the PhLP-3 protein in Drosophila. I hypothesize that DmPhLP-3 (also referred to as CG4511) plays a significant role in the development of germ line cells. I performed a spin purification of DmPhLP-3 to ensure that the protein was being expressed. I performed a large-scale stepwise purification using a gravity column. I successfully cloned, expressed, and purified truncated version of Drosophila PhLP-3 (DmPhLP-3 ΔH) in the stepwise purification.