Major
Biology
Anticipated Graduation Year
2024
Access Type
Open Access
Abstract
As Plasmodium transitions between humans and mosquitoes, it reorganizes its cytoskeleton between developmental stages. Here we present the novel Plasmodium thioredoxin-like protein 1 (TrxL-1). We hypothesize that TrxL-1 plays a role in subpellicular microtubule organization during ookinete stage development. To further study the role of TrxL-1, we aim to produce TrxL-1 specific antibodies for immunoflourescence assay. To this end we present here the cloning, expression, and purification of recombinant TrxL-1 of Plasmodium berghei. After transforming a vector containing the TrxL-1 gene into M15 protein expression cells, we performed protein purification using affinity chromatography under urea-dependent denaturing conditions.
Faculty Mentors & Instructors
Dr. Stefan Kanzok, Bioinformatics
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Expression and purification of the novel Thioredoxin-like protein 1 of the malaria parasite Plasmodium
As Plasmodium transitions between humans and mosquitoes, it reorganizes its cytoskeleton between developmental stages. Here we present the novel Plasmodium thioredoxin-like protein 1 (TrxL-1). We hypothesize that TrxL-1 plays a role in subpellicular microtubule organization during ookinete stage development. To further study the role of TrxL-1, we aim to produce TrxL-1 specific antibodies for immunoflourescence assay. To this end we present here the cloning, expression, and purification of recombinant TrxL-1 of Plasmodium berghei. After transforming a vector containing the TrxL-1 gene into M15 protein expression cells, we performed protein purification using affinity chromatography under urea-dependent denaturing conditions.