Major
Biology
Anticipated Graduation Year
2023
Access Type
Open Access
Abstract
This project aims to explore the role of Hedgehog signaling in craniofacial development in the lizard Anolis sagrei as there is a gap in knowledge regarding craniofacial development of lizards. We hypothesized that inhibition of Hedgehog signaling in A. sagrei will result in disrupted formation to the midline facial structures, specifically the premaxilla. Stage matched embryos were administered different concentrations of the chemical cyclopamine to knock down signaling at different stages. We acquired frontal and lateral microscopic and micro-CT scanned images to assess morphology. The next phase of our experiment includes in-situ experiments to detect RNA expression of Hedgehog signaling.
Faculty Mentors & Instructors
Dr. Thomas Sanger, Dr. Marta Marchini
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Knockdown of Hedgehog signaling pathway contributes to midline craniofacial development and facial elongation in the lizard Anolis sagrei
This project aims to explore the role of Hedgehog signaling in craniofacial development in the lizard Anolis sagrei as there is a gap in knowledge regarding craniofacial development of lizards. We hypothesized that inhibition of Hedgehog signaling in A. sagrei will result in disrupted formation to the midline facial structures, specifically the premaxilla. Stage matched embryos were administered different concentrations of the chemical cyclopamine to knock down signaling at different stages. We acquired frontal and lateral microscopic and micro-CT scanned images to assess morphology. The next phase of our experiment includes in-situ experiments to detect RNA expression of Hedgehog signaling.