Major
Biology
Anticipated Graduation Year
2023
Access Type
Open Access
Abstract
This research project is a contribution to the Design2Data (D2D) database. Based at UC Davis, D2D is a nationwide project with the goal of teaching an AI system to predict protein function based on structure using data from hundreds of b-Glucosidase B (BglB) mutants. We analyzed the histidine to asparagine mutation at position 328 through kinetic and thermostability assays, as well as SDS-PAGE and Western blotting to assess purity. Preliminary modeling using Rosetta software suggests the H328N BglB mutant will have little difference when compared to the wild-type enzyme.
Faculty Mentors & Instructors
Emma Feeny, Ph. D
Supported By
Design 2 Data
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Analysis of the Effects of H328N Mutation on Beta-Glucosidase B Catalytic Efficiency and Thermostability
This research project is a contribution to the Design2Data (D2D) database. Based at UC Davis, D2D is a nationwide project with the goal of teaching an AI system to predict protein function based on structure using data from hundreds of b-Glucosidase B (BglB) mutants. We analyzed the histidine to asparagine mutation at position 328 through kinetic and thermostability assays, as well as SDS-PAGE and Western blotting to assess purity. Preliminary modeling using Rosetta software suggests the H328N BglB mutant will have little difference when compared to the wild-type enzyme.