Major
Forensic Science
Anticipated Graduation Year
2023
Access Type
Open Access
Abstract
Acid phosphatase (AP), a test used in forensic cases, indicates the presence of semen. AP is also present in fungi. The aim of this project was to further evaluate the level of enzymatic activity in fungi. In this work, a spectrophotometric quantitative method was developed to test for enzymatic activity. Samples were evaluated in the wavelength range 350-600 using the Genesys 10UV spectrophotometer. The highest absorbance wavelength, 368, was used to evaluate enzymatic activity.
Faculty Mentors & Instructors
Wendy Gruhl, MS, Forensic Science
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Fungal Species Cross-Reactivity in Semen Testing (Acid Phosphatase)
Acid phosphatase (AP), a test used in forensic cases, indicates the presence of semen. AP is also present in fungi. The aim of this project was to further evaluate the level of enzymatic activity in fungi. In this work, a spectrophotometric quantitative method was developed to test for enzymatic activity. Samples were evaluated in the wavelength range 350-600 using the Genesys 10UV spectrophotometer. The highest absorbance wavelength, 368, was used to evaluate enzymatic activity.