Major
Chemistry
Anticipated Graduation Year
2024
Access Type
Open Access
Abstract
Better understanding the means by which bacteria acquire antibiotic resistance is a critical public health priority. We sought to determine the effect of environmental conditions on the evolution of antibiotic resistance, investigating the impact of carbon-limited or nitrogen-limited liquid media on the experimental evolution of Escherichia coli under the selection pressure of ampicillin. Differences in fitness between ancestral E. coli and evolved populations were measured via a fitness competition in which we mixed the populations within the same flask, forcing them to compete for resources.
Faculty Mentors & Instructors
Dr. Caroline Turner, Assistant Professor, Department of Biology
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Determining the relative fitness of E. coli populations evolved in carbon-limited or nitrogen-limited media in the presence of ampicillin
Better understanding the means by which bacteria acquire antibiotic resistance is a critical public health priority. We sought to determine the effect of environmental conditions on the evolution of antibiotic resistance, investigating the impact of carbon-limited or nitrogen-limited liquid media on the experimental evolution of Escherichia coli under the selection pressure of ampicillin. Differences in fitness between ancestral E. coli and evolved populations were measured via a fitness competition in which we mixed the populations within the same flask, forcing them to compete for resources.