Major
Molecular Biology
Access Type
Open Access
Abstract
The goal of this project is to accomplish the cloning of the gene Thioredoxin-reductase 2 (TrxR2) obtained from the male gDNA of Drosophila melanogaster into a pRSET-A vector using PCR- based cloning methods. This clone will be used to express the TrxR2 protein which will be functionally analyzed to contribute functional data to protein databases, like design2data (D2D). We hypothesize that TrxR-2 will biochemically function similarly toTrxR-1 based on the mechanism of catalysis the two proteins share.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Cloning of Thioredoxin Reductase-2 from Drosophila Melanogaster for Characterization of Catalytic Activity and Thermal Stability
The goal of this project is to accomplish the cloning of the gene Thioredoxin-reductase 2 (TrxR2) obtained from the male gDNA of Drosophila melanogaster into a pRSET-A vector using PCR- based cloning methods. This clone will be used to express the TrxR2 protein which will be functionally analyzed to contribute functional data to protein databases, like design2data (D2D). We hypothesize that TrxR-2 will biochemically function similarly toTrxR-1 based on the mechanism of catalysis the two proteins share.