Major
Biology
Anticipated Graduation Year
2024
Access Type
Open Access
Abstract
Ribbon (rib) is a transcriptional factor expressed in somatic gonadal precursor cells which coalesce with primordial germ cells to form the premature gonad in Drosophila. The Mierisch lab has found supporting evidence that rib overexpression leads to an overall decrease of somatic follicle cells (SFCs), causing a premature arrest in oogenesis. This study investigates if the reduction of SFCs are linked to a decrease in mitosis when levels of Rib proteins increase. We used the Gal4/UAS system to induce overexpression of Rib in the female Drosophila gonad, and we used fluorescent immunohistochemistry to analyze the extent of SFC proliferation.
Faculty Mentors & Instructors
Dr. Jennifer Mierisch
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
The Effect of ribbon Overexpression on Follicle Cell Proliferation
Ribbon (rib) is a transcriptional factor expressed in somatic gonadal precursor cells which coalesce with primordial germ cells to form the premature gonad in Drosophila. The Mierisch lab has found supporting evidence that rib overexpression leads to an overall decrease of somatic follicle cells (SFCs), causing a premature arrest in oogenesis. This study investigates if the reduction of SFCs are linked to a decrease in mitosis when levels of Rib proteins increase. We used the Gal4/UAS system to induce overexpression of Rib in the female Drosophila gonad, and we used fluorescent immunohistochemistry to analyze the extent of SFC proliferation.