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Major
Environmental Science
Anticipated Graduation Year
2024
Access Type
Open Access
Abstract
Five families of DNA viruses induce the reorganization of cellular chromatin (ROCC) within their host cells. Epstein-Barr virus (EBV), a gamma herpesvirus, utilizes this process. ROCC has two distinct observable phases; type I, in which the beginning of viral DNA synthesis causes global chromatin compaction, and type II, in which the cellular chromatin becomes condensed in the periphery of the nucleus. The hypothesis tested in these experiments was whether ROCC type I precedes type II. A two-step protocol using nocodazole and 4-hydroxytamoxifen was devised to investigate this question, and the data suggest that type I precedes type II.
Community Partners
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison
Faculty Mentors & Instructors
Bill Sugden, James A. Miller Professor of Oncology American Cancer Society Research Professor, McArdle Laboratory for Cancer Research
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Sequentially ordering the reorganization of cellular chromatin in Epstein-Barr virus-infected cells
Five families of DNA viruses induce the reorganization of cellular chromatin (ROCC) within their host cells. Epstein-Barr virus (EBV), a gamma herpesvirus, utilizes this process. ROCC has two distinct observable phases; type I, in which the beginning of viral DNA synthesis causes global chromatin compaction, and type II, in which the cellular chromatin becomes condensed in the periphery of the nucleus. The hypothesis tested in these experiments was whether ROCC type I precedes type II. A two-step protocol using nocodazole and 4-hydroxytamoxifen was devised to investigate this question, and the data suggest that type I precedes type II.