Presenter Information

John Michael Van DusenFollow

Major

Biology

Anticipated Graduation Year

2020

Access Type

Open Access

Abstract

The urinary microbiome is one of the least studied sites in humans. In order to study and characterize its inhabitants, new techniques for specimen isolation and DNA extraction must be developed. Here, a new protocol is proposed that is designed to collect both bacteria and viruses from the same urine sample. This removes the need for dedicated concentration and amplification steps, saving time and money. It also allows for a more complete catalogue of a sample's inhabitants. DNA extraction and Viral concentration efficiency were tested using bacteriophages obtained from our lab, combined with lab strains of Streptococcus and E. coli.

Faculty Mentors & Instructors

Dr, Catherine Putonti - Department of Bioinformatics

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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Testing a New Protocol for Extracting Microbial DNA from Urine Samples

The urinary microbiome is one of the least studied sites in humans. In order to study and characterize its inhabitants, new techniques for specimen isolation and DNA extraction must be developed. Here, a new protocol is proposed that is designed to collect both bacteria and viruses from the same urine sample. This removes the need for dedicated concentration and amplification steps, saving time and money. It also allows for a more complete catalogue of a sample's inhabitants. DNA extraction and Viral concentration efficiency were tested using bacteriophages obtained from our lab, combined with lab strains of Streptococcus and E. coli.