Major
Molecular Biology
Anticipated Graduation Year
2021
Access Type
Restricted Access
Abstract
The 2014 Ebola virus outbreak was a health crisis with global repercussions and underscores the need for rapid and inexpensive diagnostics to detect filovirus infection. We used a ribosomal display method to rapidly generate single-chain antibodies (scFv’s) for use in diagnostics against all six pathogenic filoviruses from immunized EBOV virus-like particles (VLPs). This method is inexpensive, rapid, and can be used to quickly develop repertoires of high-affinity antibodies for detection of a broad set of filovirus GPs, which demonstrates their potential use in the development of a new generation of rapid diagnostic immunoassays.
Community Partners
University of New Mexico
Faculty Mentors & Instructors
Adinarayana Kunamneni PhD Dept. of Medicine; Ravi Durvasula MD Dept. of Medicine; James Lodolce PhD Dept. of Biology
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Ribosome Display for the Generation and Selection of a Panel of Pan-Filovirus Single-Chain Antibodies as Diagnostics
The 2014 Ebola virus outbreak was a health crisis with global repercussions and underscores the need for rapid and inexpensive diagnostics to detect filovirus infection. We used a ribosomal display method to rapidly generate single-chain antibodies (scFv’s) for use in diagnostics against all six pathogenic filoviruses from immunized EBOV virus-like particles (VLPs). This method is inexpensive, rapid, and can be used to quickly develop repertoires of high-affinity antibodies for detection of a broad set of filovirus GPs, which demonstrates their potential use in the development of a new generation of rapid diagnostic immunoassays.