Major

Biology

Anticipated Graduation Year

2020

Access Type

Open Access

Abstract

In-situ hybridization is a valuable tool in the field of genetics and developmental biology. The first step to knowing the function of any gene of interest is to find out where it is being expressed. In-situ hybridization uses a series of reactions, typically over the course of a few days, to achieve specific staining that can be clearly visualized under a microscope. These stains indicate where the gene is being expressed. Using model organisms in such experiments, such as danio rerio (zebrafish), allows for high volume results in a short amount of time at minimal expense. Our zebrafish model organism has proved itself as a good candidate for gene elucidation studies such as this one, where we attempt to visualize the expression of Col2a1b in the developing embryo.

Faculty Mentors & Instructors

Rodney Dale, Ph.D, Department of Biology

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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The Expression of the col2a1b Gene During Zebrafish Embryogenesis

In-situ hybridization is a valuable tool in the field of genetics and developmental biology. The first step to knowing the function of any gene of interest is to find out where it is being expressed. In-situ hybridization uses a series of reactions, typically over the course of a few days, to achieve specific staining that can be clearly visualized under a microscope. These stains indicate where the gene is being expressed. Using model organisms in such experiments, such as danio rerio (zebrafish), allows for high volume results in a short amount of time at minimal expense. Our zebrafish model organism has proved itself as a good candidate for gene elucidation studies such as this one, where we attempt to visualize the expression of Col2a1b in the developing embryo.