Major
Neuroscience
Access Type
Restricted Access
Abstract
Neuropeptide Y (NPY) is an appetite stimulating signal whose overexpression has been linked to obesity. Much remains unknown about the cascades regulating NPY transcription, translation, and release. Here, we look for potential regulators of the Drosophila NPY homolog, neuropeptide F (NPF).We used single-cell RNA sequencing to identify CG5945, a previously uncharacterized protein, as upregulated in the fed state. CG5945 knockdown targeted to NPF cells results in increased food intake, starvation resistance, and NPF expression. This identifies CG5945 as a novel inhibitor of the NPF, and thus orexigenic, signaling cascade.
Faculty Mentors & Instructors
Jennifer Beshel, Assistant Professor, Department of Biology
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
A novel inhibitor of feeding in Drosophila
Neuropeptide Y (NPY) is an appetite stimulating signal whose overexpression has been linked to obesity. Much remains unknown about the cascades regulating NPY transcription, translation, and release. Here, we look for potential regulators of the Drosophila NPY homolog, neuropeptide F (NPF).We used single-cell RNA sequencing to identify CG5945, a previously uncharacterized protein, as upregulated in the fed state. CG5945 knockdown targeted to NPF cells results in increased food intake, starvation resistance, and NPF expression. This identifies CG5945 as a novel inhibitor of the NPF, and thus orexigenic, signaling cascade.