Major

Molecular Biology

Anticipated Graduation Year

2021

Access Type

Open Access

Abstract

We set out to characterize the expression of col2a1b, the zebrafish ortholog of the human COL2A1. In zebrafish, the col2a1a gene has been shown to be expressed in both chondrocytes and perichondrial cells, whereas our preliminary work suggests col2a1b is expressed only in the perichondrium of cartilage elements; thus, col2a1b collagen promotes cartilage development and ossification of chondrocytes. Our lab is interested in understanding the differential expression of the two homologs. In this report, we document the complete spatiotemporal expression of the zebrafish col2a1b mRNA during the first 5 days of development via in situ hybridization.

Faculty Mentors & Instructors

Dr. Rodney M. Dale, Associate Professor, Biology

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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Expression of mRNA of the col2a1b gene during zebrafish embryogenesis

We set out to characterize the expression of col2a1b, the zebrafish ortholog of the human COL2A1. In zebrafish, the col2a1a gene has been shown to be expressed in both chondrocytes and perichondrial cells, whereas our preliminary work suggests col2a1b is expressed only in the perichondrium of cartilage elements; thus, col2a1b collagen promotes cartilage development and ossification of chondrocytes. Our lab is interested in understanding the differential expression of the two homologs. In this report, we document the complete spatiotemporal expression of the zebrafish col2a1b mRNA during the first 5 days of development via in situ hybridization.