Major
Biology
Anticipated Graduation Year
2026
Access Type
Open Access
Abstract
We are investigating how nutrient-limitation affects the evolution of antibiotic resistance in Escherichia coli. We evolved E. coli for 24 days in nitrogen- or carbon-limited environments with and without tobramycin. Many nitrogen-limited strains that evolved with tobramycin did not develop antibiotic resistance and went extinct. Surprisingly, fitness competitions showed that nitrogen-limited populations had an even larger fitness advantage in nitrogen-limited environments with tobramycin than in their evolutionary environments. These populations had a mutation in either gene glnL or glnK. We isolated each mutation in a clone and ran fitness assays to investigate how glnL and glnK mutations affected tobramycin resistance.
Faculty Mentors & Instructors
Caroline Turner, Ph.D, Assistant Proffessor, Department of Biology
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Nitrogen Limitation Impacts the Evolution of Tobramycin Resistance in E.coli
We are investigating how nutrient-limitation affects the evolution of antibiotic resistance in Escherichia coli. We evolved E. coli for 24 days in nitrogen- or carbon-limited environments with and without tobramycin. Many nitrogen-limited strains that evolved with tobramycin did not develop antibiotic resistance and went extinct. Surprisingly, fitness competitions showed that nitrogen-limited populations had an even larger fitness advantage in nitrogen-limited environments with tobramycin than in their evolutionary environments. These populations had a mutation in either gene glnL or glnK. We isolated each mutation in a clone and ran fitness assays to investigate how glnL and glnK mutations affected tobramycin resistance.