Presenter Information

Dawid MaciorowskiFollow

Major

Molecular Biology

Anticipated Graduation Year

2021

Access Type

Restricted Access

Abstract

The 2014 Ebola virus outbreak was a health crisis with global repercussions and underscores the need for rapid and inexpensive diagnostics to detect filovirus infection. We used a ribosomal display method to rapidly generate single-chain antibodies (scFv’s) for use in diagnostics against all six pathogenic filoviruses from immunized EBOV virus-like particles (VLPs). This method is inexpensive, rapid, and can be used to quickly develop repertoires of high-affinity antibodies for detection of a broad set of filovirus GPs, which demonstrates their potential use in the development of a new generation of rapid diagnostic immunoassays.

Community Partners

University of New Mexico

Faculty Mentors & Instructors

Adinarayana Kunamneni PhD Dept. of Medicine; Ravi Durvasula MD Dept. of Medicine; James Lodolce PhD Dept. of Biology

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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Ribosome Display for the Generation and Selection of a Panel of Pan-Filovirus Single-Chain Antibodies as Diagnostics

The 2014 Ebola virus outbreak was a health crisis with global repercussions and underscores the need for rapid and inexpensive diagnostics to detect filovirus infection. We used a ribosomal display method to rapidly generate single-chain antibodies (scFv’s) for use in diagnostics against all six pathogenic filoviruses from immunized EBOV virus-like particles (VLPs). This method is inexpensive, rapid, and can be used to quickly develop repertoires of high-affinity antibodies for detection of a broad set of filovirus GPs, which demonstrates their potential use in the development of a new generation of rapid diagnostic immunoassays.