Major
Biology
Anticipated Graduation Year
2020
Access Type
Open Access
Abstract
The Drosophila species’ 4th chromosome has maintained its heterochromatin properties. The goal of the project was to annotate and understand the regulation of the DNA in heterochromatin environment. We annotated contig 52, part of the 4th chromosome of species D. ananassae. The contig 52 contains 3 genes: CG33978 (5 isoforms), CG32006, and bip2-PA. The findings of the bip2 gene annotation showed a disparity between D. ananassae and D. melanogaster species. Although annotating the bip2-PA gene in the genome browser with the evidence provided by Blastx and the gene record finder did not match with what we observed while annotating each CDS’s. In D. ananassae, exon#3 is split into two and are transcribed individually. Figure 6 shows D. mel chain evidence for only one exon but there are two exons based on RNA-seq and RAMPAGE data.
Community Partners
Genome education partnership
Faculty Mentors & Instructors
Dr. Jennifer Mierisch
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Genome Annotation in Drosophila ananassae
The Drosophila species’ 4th chromosome has maintained its heterochromatin properties. The goal of the project was to annotate and understand the regulation of the DNA in heterochromatin environment. We annotated contig 52, part of the 4th chromosome of species D. ananassae. The contig 52 contains 3 genes: CG33978 (5 isoforms), CG32006, and bip2-PA. The findings of the bip2 gene annotation showed a disparity between D. ananassae and D. melanogaster species. Although annotating the bip2-PA gene in the genome browser with the evidence provided by Blastx and the gene record finder did not match with what we observed while annotating each CDS’s. In D. ananassae, exon#3 is split into two and are transcribed individually. Figure 6 shows D. mel chain evidence for only one exon but there are two exons based on RNA-seq and RAMPAGE data.